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. 2021 Jan 7;7:578892. doi: 10.3389/fmolb.2020.578892

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Copyright © 2021 Lin, Guo, Liu and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sensitivity of the PSR-LFB assay for HBV detection. (A–C) Analysis the PSR amplicons by AGE, SGI, and LFB assay, respectively. (D,E) Amplification results of LAMP assay by AGE and SGI method. (F) Detection of HBV by qPCR. M: DNA maker DL8000. Lanes/tubes/biosensors/fluorescence signals 1–9: 5.4 × 10⁶, 5.4 × 10⁵, 5.4 × 10⁴ 5.4 × 10³, 5.4 × 10², 5.4 × 10¹, 5.4 × 10⁰, 5.4 × 10^–1, 5.4 × 10^–2 copies/mL, respectively. N: negative control.