FIGURE 1.

Resveratrol treatment stimulates lipid accumulation in human muscle cells. Human myoblasts were cultured and differentiated and incubated with or without 50 ng/ml resveratrol. (a–c) Time course analysis for LD area (a), LD number (b) and LD size (c). Data are obtained from one donor, from multiple wells. Data are presented as mean fold change ± SEM (n = 6 time points); analysed by two‐way ANOVA. (d–f) Effect of resveratrol on total LD area (d), total LD number (e) and average LD size (f) after 48 h incubation. Data are obtained from three donors, from multiple wells in fixed cells. Data are presented as mean ±SEM (n = 3). (g–i) After 48 h incubation, cells were fixed and nuclei were stained with DAPI (blue) and neutral lipids with Bodipy 493/503 (green). Bodipy area/nuclei was determined with or without resveratrol. Data are obtained from three donors and 2–4 repeated experiments. Data are presented as mean fold change ± SEM (n = 9). *Significantly different from control (p < 0.05, paired t‐test). Images are representative for (g) incubated without (h) and with resveratrol (i). Scale bar = 40 µm