Table 1.
Effects of silencing Numb, Nedd4-2 and Eps15 on gating properties of sodium channels in DRG neurons from WT and CRMP2K374A/K374A female mice
| WT | CRMP2K374A/K374A | |||
|---|---|---|---|---|
| siRNA | ||||
| Control | Numb | Control | Numb | |
| Activation | ||||
| V1/2 | − 13.2±0.5 (15) | − 17.9 ± 0.7 (11) | − 17.8 ± 1.2 (9) | 17.5 ± 0.9 (15) |
| k | 5.6 ± 0.4 (15) | 6.0 ± 0.6 (11) | 8.6 ± 1.1 (9) | 7.6 ± 0.8 (15) |
| Inactivation | ||||
| V1/2 | − 47.1 ± 1.4 (13) | − 56.8 ± 1.2 (12) | − 56.2 ± 1.7 (10) | − 47.8 ± 2.4 (15) |
| k | − 12.3 ± 1.4 (13) | − 10.5 ± 1.1 (12) | − 14.8 ± 1.7 (10) | − 17.9 ± 2.6 (15) |
| Control | Nedd4-2 | Control | Nedd4-2 | |
| Activation | ||||
| V1/2 | − 18.5 ± 0.7 (12) | − 17.3 ± 1.0 (15) | − 19.2 ± 1.0 (12) | − 19.4 ± 0.7 (19) |
| k | 5.9 ± 0.6 (12) | 8.2 ± 0.9 (15) | 8.5 ± 1 (12) | 5.8 ± 0.6 (19) |
| Inactivation | ||||
| V1/2 | − 47.3 ± 2.5 (11) | − 47.5 ± 2.5 (15) | − 61.2 ± 1.7 (13) | − 52.0 ± 2.0 (20) |
| k | − 17.0 ± 2.7 (11) | − 18.7 ± 2.8 (15) | − 11.7 ± 1.6 (13) | − 18.2 ± 2.2 (20) |
| Control | Eps15 | Control | Eps15 | |
| Activation | ||||
| V1/2 | − 18.5 ± 0.7 (12) | − 15.6 ± 1.3 (13) | − 19.2 ± 1.0 (12) | − 23.8 ± 0.7 (17) |
| k | 5.9 ± 0.6 (12) | 9.0 ± 1.2 (13) | 8.5 ± 1.0 (12) | 6.6 ± 0.6 (17) |
| Inactivation | ||||
| V1/2 | − 47.3 ± 2.5 (11) | − 45.7 ± 2.4 (11) | − 61.2 ± 1.7 (13) | − 57.8 ± 1.6 (23) |
| k | − 17.0 ± 2.7 (11) | − 16.4 ± 2.6 (11) | − 11.7 ± 1.6 (13) | − 16.4 ± 1.6 (23) |
Values are means ± SEM calculated from fits of the data from the indicated number of individual cells (in parentheses) to the Boltzmann equation; V1/2 midpoint potential (mV) for voltage-dependent activation or inactivation; k, slope factor. These values pertain to Figs. 3, 4 and 5. Data were analyzed with one-way ANOVA with Dunnett’s post hoc test. No statistically significant differences are reported