Table 1.

Comparison of k_cat values for human prothrombinase activity

Source	Prothrombinase complex surface	PL Conc.	FXa Conc.	FVa Conc.	$\frac{\text{kcat}}{{\text{min}}^{-1}}$
Moyer et al [52]	PC/PS (75/25)	5.0 μM	5.0 nM	5.0 nM	480
Deguchi et al [14]	SkM	-	0.2 nM	5.0 nM	490
Zilberman-Rudenko et al [16]	CM	-	0.2 nM	5.0 nM	371
Deguchi et al [14]	PC/PS (80/20)	20 μM	0.2 nM	5.0 nM	396
Krishnaswamy et al [51]	PC/PS (75/25)	30 μM	1 nM	4.1 nM	1,344
Bunce et al [53]	Platelet (1×108/mL)		0.1 nM	10 nM	560

kcat (V_max/[FXa]) values for SkM, CM, phospholipids, and activated platelets are summarized. For comparisons in this Table, note that experimental conditions included modest variations, inter alia, in assay buffer contents (Tris vs. HEPES, 2 mM vs. 5 mM CaCl₂ concentrations, etc.), different temperatures and different phospholipid (PC/PS) ratios. See references [14, 51–53] for details about differences in assay contents and conditions. This Table is modified from previous papers [14, 16]. Abbreviations: PL, phospholipid; FXa, Factor Xa; FVa, Factor Va; PC/PS, phosphatidylcholine/phosphatidylserine.