Skip to main content
. 2021 Jan 1;41(1):51–61. doi: 10.1002/cac2.12121

FIGURE 7.

FIGURE 7

atRA inhibits B7‐H6 transcription by down‐regulating c‐Myc. A‐B. U‐937 cells were transfected with c‐Myc siRNA (si‐c‐Myc) or control siRNA (si‐Control) and harvested for 24 h. c‐Myc mRNA and B7‐H6 mRNA expression were analyzed by real‐time PCR (A). B7‐H6 cell‐surface expression was analyzed by flow cytometry (B). C. U‐937 cells were treated with 10 μmol/L atRA or left untreated (Control) for 24 h or 48 h, and c‐Myc mRNA expression was analyzed by real‐time PCR. D. U‐937 cells were treated with atRA or left untreated (Control) for 24 h, and then c‐Myc protein expression was analyzed by western blotting. E. THP‐1 cells were treated with atRA at various concentrations for 24 h and c‐Myc mRNA was analyzed by real‐time PCR. Data were analyzed for significance by the unpaired t test or the one‐way ANOVA test. Results are representative of at least three independent experiments with a minimum of two technical replicates and are expressed as the mean±SEM. ****, P <0.0001.