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. 2021 Jan 21;17(1):e1009233. doi: 10.1371/journal.pgen.1009233

Fig 2. Bone formation was impaired in Runx1f/fCol1α1-Cre and Runx1f/fTwist2-Cre mice, with significantly reduced expression of osteoblast genes.

Fig 2

(A, B) H&E stain of (A) 2-month-old and (B) 8-month-old Runx1f/fCol1α1-Cre mice femurs. (C) H&E stain of 6-month-old Runx1f/fTwist2-Cre mice femurs. (D) Immunostaining using antibodies against Runx2, Opn, Atf4, and Osx in newborn Runx1f/fCol1α1-Cre (f/f/Δ) and Runx1f/f (f/f) mouse femurs. (E) quantification of immunostaining-positive cells in D. (F) Immunostaining using antibodies against Runx1, Opn, and Osx in newborn Runx1f/fTwist2-Cre (f/f/Δ) and Runx1f/f (f/f) mouse femurs. (G) quantification of immunostaining-positive cells in F. Results are expressed as mean ± SD, n = 4 in each group. N.S, not significant; **p <0.01.