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. 2021 Jan 21;17(1):e1009233. doi: 10.1371/journal.pgen.1009233

Fig 8. Overexpression of Runx1 increases osteoblasts and decreases adipocytes and osteoblast markers levels in vitro.

Fig 8

(A) Overexpression of GFP in 14 days induced calvarial cells by retrovirus. (B) Retrovirus-mediated overexpression of Runx1 in 14 days induced calvarial cells. Osteoblast differentiation was analyzed by ALP activity on day 14, while adipocytes were analyzed by Oil red staining on day 14. (C) Western blot was performed on day 14 to evaluate the protein levels of Runx1, Runx2, Atf4, Osx, Opn, and Ocn. GAPDH is shown as a control. (D) Quantification of protein levels from western blot in C. (E) q-PCR was conducted on day 14 to evaluate the mRNA levels of Runx1, Runx2, Atf4, Bmp7, Alk3, Osx, Col1α1, C/ebpα and Pparg. All data are presented as mean ± SD, n = 4, NS denotes not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.