Fig 2. BMDM from male S. mansoni infected HFD ApoE-/- mice have increased TCA cycle usage with significantly increases free fatty acids and reduced cholesterol esters.
A-E) Mϕ were differentiated from bone marrow of 10-week infected animals with M-CSF in a 7-day culture in normal glucose and then switched to 13C-labeled glucose for 24 hours. F-I) Mϕs were differentiated with M-CSF in a 7-day culture and then total cellular lipids were extracted and analyzed via LC-MS based lipidomic analysis. B) Volcano plot of significantly altered species in uninfected: infected male BMDM C) The variable importance in projections (VIP) scores from uninfected males in comparison to infected males D-E) Box whiskers plots (10–90 percentile) of relative abundance of normalized cholesterol ester and the indicated free fatty acid species, data are normalized to sum and treated with pareto scaling, each dot is a single species. Data are representative of 3 experiments with 4–6 mice per group in each experiment.