Fig 3. S. mansoni infection induces fatty acid oxidation and lowers chronic inflammatory factors in male ApoE-/- male mice.
(A) Macrophages from control and S. mansoni infected ApoE-/- mice were differentiated with M-CSF in a 7-day culture with normal glucose and serum, and then switched to 13C-labeled palmitate in dialyzed serum for 36 hours followed by metabolic flux analysis (B-C) BMDM were differentiated with M-CSF in a 7-day culture with normal glucose and serum, and then switched to glucose limited media with Palmitate-BSA as the carbon source followed by Seahorse analysis of OCR and SRC in the presence of the CPT-1 inhibitor etomoxir. (D) Schematic of glucose, glutamine, and palmitate inputs into the TCA cycle. (E-H) Quantification of inflammatory mediators in BMDM supernatants 24 hours post stimulation with LPS. Data are representative of two biologically independent experiments.