Fig 7. S. mansoni infection modulates bone marrow myeloid progenitors in a sex-dependent manner.

(A) Total cell counts from bone marrow cells utilizing trypan-blue discrimination of apoptotic cells (B) Percentages of CMP (Lin^-CD127^-c-Kit⁺Sca-1^-CD34⁺CD16/32^low) and GMP (Lin^-CD127^-c-Kit⁺Sca-1^-CD34⁺CD16/32^hi) (C) CMP cell counts in bone marrow ApoE^-/- (D) GMP cell counts 10 week post infection in ApoE^-/- mice (E) Flow cytometry analysis of MDP defined as Lin^-c-Kit⁺Sca-1^-FcγR^lowCD34⁺CD115⁺ (F) MDP cell counts in ApoE^-/-uninfected and infected male and female mice. (G) Ki67 expression in CMP, GMP, and MDP in bone marrow from 10-week infected and control male and female ApoE^-/- mice on HFD. (H) Frequency and total number of LMPP (defined as Lin^-CD127^-c-Kit⁺Sca-1⁺CD34⁺flt3⁺). (G) Diagram of mouse bone marrow progenitors/differentiation pathways. Graphs are representative from experiments that were performed 2–3 times with n>4. Statistical analysis was done using Brown Forsythe and Welch ANOVA.