Figure 2.

Representative wristband extract-induced inhibited cell viability. HEK293T cells were transfected with plasmids and induced with wristband extracts and/or control chemicals as described in the Materials and Methods. Representative dose response curves are provided based on concentration of extraction in contact with the cells (0.1% = 1000-fold dilution of the extract into the exposure media). Cells were induced for approximately 18 h, after which cell viability was assessed by two different measures: via significant reduction in β-gal activity as per constitutively active promoter plasmid relative to vehicle/solvent control (A) and via significant release of LDH relative to a cytotoxic control (B). Data presented as mean ± SEM from two or three independent experiments and four technical replicates of each concentration within each.