Figure 4. Hydroxysafflor yellow A inhibited protein expression of proinflammatory cytokine and TLR4/NF-kB protein in ipsilateral hippocampus of TBI rats. (a) Representative bands of western blot for IL-1β, IL-6, TNF-α, TLR4 and NF-κBprotein levels in sham, vehicle and HSYA group 24 h after TBI; (b-f) quantification of IL-1β, IL-6, TNF-α, TLR4 andNF-κB proteins are presented. Quantified results were revised by β-actin expression. N = 4 per group; data are presented as mean ± SD;