Figure 1. Decrease in the relative abundance (analyzed as mol%) of longer and more unsaturated fatty acids in VLDL₁ and VLDL₂ TGs in homozygous carriers of the TM6SF2 E167K genetic variant.

Lipids were extracted from VLDL₁ and VLDL₂, and the number of double bonds in fatty acids (i.e., degree of unsaturation) in TGs containing 48 to 58 carbon molecules was determined. The colors indicate the ratio of the individual lipid species in carriers of the TM6SF2 E167K genetic variant (n = 10) versus controls (n = 10). A linear mixed model with number of double bonds as fixed effect and subject as random effect was used to test for a linear effect of the number of double bonds, within fixed chain lengths. Data were normalized to mean of control group; P values correspond to tests of the linear effect of double bonds differing from 0. *P < 0.05.