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. 2020 Dec 17;5(24):e142272. doi: 10.1172/jci.insight.142272

Figure 6. Inhibition of miR-376b attenuates LPS-induced septic AKI in mice.

Figure 6

C57BL/6 male mice were given 20 mg/kg anti–miR-376b or scrambled sequence oligonucleotides (negative control [NC]) and then injected with 10 mg/kg LPS or normal saline (NS) as control. Kidney tissues were collected at 24 hours after LPS injection. (A) Serum creatinine. (B) BUN. (C) Representative images of H&E staining. Scale bar: 100 μm. (D) Representative images of TUNEL staining. Scale bar: 100 μm. (E) Quantification of TUNEL-positive cells. (F) Immunoblot analysis of active caspase-3 in kidney cortical tissues. GAPDH was used as loading control. (G–I) qPCR analysis of Mcp1, Il6, and Tnfa mRNA. All quantitative data were expressed as mean ± SD (n = 4 or n = 6 in A, B, and G–I and n = 10 in E, 1-way ANOVA with Tukey’s multiple comparisons test), *P < 0.05.