Figure 1.

lnc-CCNL1-3:1 is upregulated in granulosa cells of women with PCOS

(A) The expression level of CCNL in 43 women with PCOS and 41 healthy controls. The expression level was detected via quantitative real-time PCR. The first ΔCt normalization was to β-actin, and the second ΔCt normalization was to the control group. (B) Bar graph showing CCNL vector-mediated overexpression efficiency. KGN cells or hLGCs stably expressing empty vector (Enter) or CCNL overexpression vectors (CCNL) were cultured 72 h and then subjected to quantitative real-time PCR and normalized against β-actin. Error bars represent SDs of at least three independent experiments. ∗∗∗∗p < 0.0001 corresponds to two-tailed Student’s tests.