Figure 4.

lnc-CCNL1-3:1 suppressed cell glucose uptake in vitro

(A) The expression level of CCNL was correlated with BMI in patients with PCOS (p = 0.0070). (B) The higher HOMA-IR values had a higher expression level of CCNL (p = 0.0784, tertile I versus II; p = 0.0017, tertile I versus III; p = 0.0267, tertile II versus III). (C and D) Basal and insulin-stimulated glucose uptake levels were reduced in CCNL-transfected KGN cells and hLGCs. The cells were serum starved for 12 h following insulin stimulation (100 nM, 20 min) or PBS as control before the detection. (E) Western blotting analysis to measure relative protein expression (GLUT4, IRS1) from KGN cells and hLGCs after transfection with vectors for 72 h. Error bars represent SDs of at least three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 correspond to two-tailed Student’s tests.