Figure 4.

Synthetic tRF^GlnCTG mimetics enhanced the proliferation and migration of the cultured rat VSMCs, whereas the inhibitor reduced the proliferation and migration

(A) The transfection efficacy of tRF^GlnCTG mimetics and inhibitors was validated by qPCR. (B) Cell counting kit-8 (CCK-8) assay showed that the transfection of tRF^GlnCTG mimetics increased DNA synthesis in VSMCs, and the inhibitors reduced it. (C) EdU incorporation assay demonstrated that the tRF^GlnCTG mimetics elevated the cell viability, and the inhibitors weakened it. (D) Transwell assay revealed that tRF^GlnCTG mimetics induced the migration of VSMCs, and the inhibitors reduced it. (E) Wound-closure assay showed that mimetics accelerated the wound-healing rate, and the inhibitors declined it (n = 5). Data are expressed as means ± SEM. ∗p < 0.05, ∗∗p < 0.01.