Figure 2.
NSD2 upregulation leads to cisplatin resistance in ESCC cells
(A) Western blot analysis of NSD2 and H3K36me2 protein levels in ESCC cells transfected with empty vector or NSD2-expressing plasmids. (B) Bar graphs show the cisplatin IC50 values in ESCC cells transfected with empty vector or NSD2-expressing plasmids. (C) Cell proliferation was determined by EdU incorporation assay after treatment with or without cisplatin for 72 h. Left, representative merged images of EdU incorporation (red) and nuclear staining (blue). Scale bar, 50 μm. Right, quantification of EdU-positive cells. ∗p < 0.05. ns indicates no significance. (D) Measurement of apoptosis by Annexin V/PI staining in the transfected cells after 10 μM cisplatin treatment. (E) Colony formation assay. NSD2 overexpression enhanced long-term survival of ESCC cells after treatment with 10 μM cisplatin. Right, quantitative analysis of colonies. ∗p < 0.05.