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. 2020 Dec 10;23:592–602. doi: 10.1016/j.omtn.2020.12.007

Figure 3.

Figure 3

NSD2 depletion increases cisplatin sensitivity in ESCC cells in vitro

(A) Western blot analysis of NSD2 protein levels in ESCC cell lines and HEsEpiC cells. (B) Analysis of NSD2 mRNA levels in EC109 cells transfected with control shRNA (shCtrl) or NSD2-targeting shRNA (shNSD2). (C) Western blot analysis of NSD2 and H3K36me2 protein levels in EC109 cells transfected with shCtrl or shNSD2. (D) Silencing of NSD2 suppressed the proliferation of EC109 cells. (E) Measurement of the cisplatin IC50 values in EC109 cells transfected with shCtrl or shNSD2. (F) Detection of apoptosis in EC109 cells transfected with shCtrl or shNSD2 after treatment with 10 μM cisplatin. (G) Measurement of NSD2 mRNA levels in resistant KYSE150 and KYSE30 cells transfected with shCtrl or shNSD2. (H) Measurement of the cisplatin IC50 values in resistant KYSE150 and KYSE30 cells transfected with shCtrl or shNSD2. ∗p < 0.05.