Figure 1.

Assessment of psoriasis symptoms induced by imiquimod in Zdhhc2 knockout mice. (A) Schematic representation of imiquimod stimulation on mice ear. (B) Zdhhc2 expression level was analyzed by qRT-PCR in ear skin of WT mice with (8 days) or without (0 days) imiquimod treatment (n = 3, mean ± SEM). (C) Zdhhc2 knockout mice were screened by PCR using tail genomic DNA of Zdhhc2^−/− mice. Results from Zdhhc2^−/− mice were shown and compared to WT mice. H₂O was used as negative control. (D) Zdhhc2^−/− mice were verified by qRT-PCR analysis (n = 3, mean ± SEM). (E) Representative pictures of WT and Zdhhc2^−/− mice ears respectively treated with imiquimod for 0, 4, and 8 days. Experiments were repeated three times involving three mice for each treatment group. (F–H) Imiquimod was applied on ear skin of WT and Zdhhc2^−/− mice for 8 consecutive days, and ear thickness, scaling, and erythema were measured on a daily basis. Scaling and erythema were scored independently from 0 to 4 (see Materials and Methods). Two representative experiments are depicted (n = 6, mean ± SEM). ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001, ^**** p < 0.0001, unpaired Student’s t-test.