TABLE 1.
Time for the first detection of transgenic parasites.
| Gene name | Gene ID | Gene edition | Construct | Tag length (bp) |
Days |
|
| Cas9e | Cas9i | |||||
| Sir2B | PF3D7_1451400 | Mutation | Shield mutations | – | 29 | 17 |
| ARP4 | PF3D7_1422800 | Knockin | ARP4-Ty1-Ribo | 390 | 28 | 15 |
| ARP6 | PF3D7_0719300 | Knockin | ARP6-HA-Ty1 | 219 | 21 | 16 |
| MTR4 | PF3D7_0602100 | Knockin | MTR4-Ty1-Ribo | 390 | 20 | 18 |
| Average days* | 24.5 | 16.5 | ||||
*P = 0.016 (unpaired two-tailed Student’s t-test). Every gene was edited by two independent repeated transfections, and the average days of the two transfections are shown in the table.