Table 2.
Performance parameters of the LC-MS/MS-method including recovery rates (n = 6 replicates) and precision
| Toxin | Matrix | LOD [μg/kg] | LOQ [μg/kg] | Level of fortification [μg/kg] | Recovery [%] | RSDr [%] | RSDR [%] |
|---|---|---|---|---|---|---|---|
| T-2 | Larvae | 4.6 | 15.3 | 75 | 107.6 | 0.1 | 6.9 |
| 187.5 | 112 | 1.3 | 7.1 | ||||
| Oat flakes | 3.3 | 11 | 75 | 101.2 | 4 | 18.5 | |
| 187.5 | 101.2 | 4 | 8.4 | ||||
| Residue | 2.1 | 7 | 75 | 115.4 | 6.1 | 8.6 | |
| 187.5 | 102.7 | 8.3 | 1.9 | ||||
| HT-2 | Larvae | 8.4 | 27.9 | 75 | 101.8 | 0.1 | 11 |
| 187.5 | 134.3 | 7.2 | 10.2 | ||||
| Oat flakes | 12 | 39.7 | 75 | 86.1 | 7.7 | 17.3 | |
| 187.5 | 79.8 | 6.4 | 10.3 | ||||
| Residue | 20.5 | 68.2 | 75 | 97.7 | 7.5 | 16.9 | |
| 187.5 | 100 | 6.5 | 8.9 | ||||
| T-2 triol | Larvae | 29.6 | 98.8 | 75 | 104.3 | 3.4 | 9.6 |
| 187.5 | 105.9 | 4.9 | 7 | ||||
| Oat flakes | 9.5 | 31.5 | 75 | 75.9 | 11.4 | 25.4 | |
| 187.5 | 72.8 | 10 | 27.1 | ||||
| Residue | 69 | 184.4 | 187.5 | 116.1 | 16.3 | 9.5 | |
| 300 | 95.8 | 3.7 | 11.5 | ||||
| T-2 tetraol | Larvae | 24.6 | 82.4 | 75 | 50.1 | 15.9 | 8.5 |
| 187.5 | 48.5 | 9.5 | 11 | ||||
| Oat flakes | 30 | 102 | 75 | 60.1 | 24.1 | 11.1 | |
| 187.5 | 59.4 | 7.4 | 14.1 | ||||
| Residue | 25 | 83.7 | 75 | 50.1 | 22.8 | 10.6 | |
| 187.5 | 50 | 7.3 | 5.8 |
LOD, limit of detection; LOQ, limit of quantification; RSDr, intraday precision was determined as relative standard deviation by 6 replicates for each sample material and concentration; RSDR, interday precision was determined as relative standard deviation with one replication on 5 consecutive days