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. 2021 Jan 8;11:603183. doi: 10.3389/fmicb.2020.603183

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Copyright © 2021 Yang, Wang, Jiang, Lin, Ou, Ullah, Hua, Chen, Lin, Hu, Zheng and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The size distribution and morphology of extracellular vesicles (EVs) secreted by Talaromyces marneffei (TM). (A) Nanoparticle tracking analysis revealed the size distribution and particle concentration of isolated EVs. (B) The EVs were observed inside TM through transmission electron microscopy (TEM; black arrow). (C) It showed the moment of TM yeast cells releasing EVs (black arrow). (B,C) Scale bar = 200 nm. (D,E) TEM identified the purified EVs’ morphology with a cup-shaped structure. (D) Scale bar = 200 nm. (E) Scale bar = 50 nm.