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. 2021 Jan 21;11:1873. doi: 10.1038/s41598-021-81518-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The nucleotide sequences and position of the final (A) ORF1ab and (B) N gene COVID-19 SARS-CoV-2 MCDA primer sets chosen in this study. Right and left arrows show sense and complementary sequences, respectively while coloured text indicates the position of primers: F1/F2 displacement primers in purple, P1/P2 primers in green, C1/C2 amplification primers in blue, D1/D2 amplification primers in yellow and R1/R2 amplification primers in red.