Table 2.
Comparison of the sensitivity and time to detection for MCDA and LAMP targeting ORF1ab from 3 independent runs.
DNA copies per reaction | MCDA average detection time (min) | MCDA reps$ | LAMP average detection time (min) | LAMP reps$ |
---|---|---|---|---|
10 | 55.3 | 1/9 | 17.1 ± 6.3 | 3/9 |
25 | 46.4 ± 15 | 3/9 | 11.8 ± 0.5 | 5/9 |
50 | 20.2 ± 9 | 8/9 | 12.2 ± 2.1* | 9/9* |
100 | 13.6 ± 3.9 | 6/9 | 10.4 ± 0.8 | 8/9 |
500 | 12.6 ± 1.3* | 9/9* | 9.7 ± 0.2 | 9/9 |
1000 | 11.2 ± 0.3 | 9/9 | 9.5 ± 0.1 | 9/9 |
10,000 | 9.7 ± 0.3 | 9/9 | 8.4 ± 0.1 | 9/9 |
*Limit of detection.
$reps: 3 runs with 3 technical replicates = 9 replicates.