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. 2021 Jan 21;11:1996. doi: 10.1038/s41598-021-81689-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Correlations between gene expression and GnRH release from the MBH after EED overexpression. (A) Scatterplot of total GnRH release vs. average GnRH pulse frequency/hour over a 4 h incubation period of MBH fragments derived from late juvenile 28-day-old female rats injected 6 days earlier with a lentiviral expressing GFP alone (n = 8) or EED plus GFP (n = 8). Best-fit linear correlation is indicated by black line. (B) Plot of partial correlations (R) of gene expression (G) as assayed by OpenArray/qPCR with total (T) GnRH release and pulse amplitude (PA) with regard to (wrt) other physiological metric. (C) Plot of total GnRH release ordered from lowest to highest (yellow squares) and regression model predictions for Ŷ_{Total Expression} = β_intercept + β_{Kdm6b expression} (red circles) and Ŷ_{Total Expression} = β_intercept + β_{Kdm6b expression} + β_{Kdm6b expression x Pulse Amplitude(PA)} (green circles). MBH number refers to one of the 16 MBH incubated in vitro. (D) Plot of total GnRH release ordered from lowest to highest (yellow squares) and regression model prediction for Ŷ_{Total Expression} = β_intercept + β_{Kdm6b expression} + β_{Kdm6b expression × Pulse Amplitude (PA)} + β_{Kdm6b expression × Pulse Amplitude (PA) × Pulse Frequency(PF)} (red circles).