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. 2021 Jan 21;11:1996. doi: 10.1038/s41598-021-81689-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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KDM6B counteracts the effects of EED. Rat R22 cells were transiently transfected with rat Eed, a human KDM6B expression vector or both. Expression of (A) rat Eed, (B) rat endogenous Kdm6b (yellow bars) and human exogenous KDM6B (orange bars) and, (C) rat Kiss1 was determined 48 h after transfection. mRNA levels were measured by qPCR. Results are expressed as fold change with respect to control-pcDNA values. a,b p < 0.001 vs pcDNA and KDM6B groups. c, d, p < 0.05 vs pcDNA and KDM6B groups. e, p < 0.05 all other groups (One Way ANOVA followed by Student-Newman–Keuls test) (n = 3 per group). N.D. = not detectable.