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. 2021 Jan 21;11:1986. doi: 10.1038/s41598-021-81433-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Optimization of (a) wash buffer volume and binding times for (b) the target antigen (cTnI) and detection antibody, (c) the target antigen (cTnI)/detection antibody complex and the capture antibody, and (d) TMB enzyme–substrate with capture antibody/antigen/detection antibody complex for the proposed ELISA protocol in the developed microfluidic platform. The error bars represent ± standard deviation of n = 3 samples.