Table 1:

Primers targeting the full ITS region (3.5 Kb) and the whole fungal operon (6 Kb)

Name	Sequence (5′-3′)	Target	Amplicon	Reference
SSU515Fngs-F	TTTCTGTTGGTGCTGATATTGCGCCAGCAACCGCGGTAA	18S-V3	3.5 Kb	Tedersoo et al. [20]
LR5-R	ACTTGCCTGTCGCTCTATCTTCTCCTGAGGGAAACTTCG	28S-D3	3.5 Kb	Tedersoo et al. [20]
SR1R-Fw	TTTCTGTTGGTGCTGATATTGCTACCTGGTTGATQCTGCCAGT	18S-V1	6 Kb	Vilgalys Lab [25]
LR12-R	ACTTGCCTGTCGCTCTATCTTCGACTTAGAGGCGTTCAG	28S-D12	6 Kb	Vilgalys Lab [25]

The Nanopore Universal Tag in bold type.