Table 2.
Results of antimicrobial in vitro activity of pure oils (O6, O14, and O19) and NLC (F6, F14, and F19), in terms of the diameter of growth inhibition zone (IZ) and the minimum inhibitory concentration (MIC) against CJ strains, in free and sessile forms (n = 3).
| Sample | free form IZ IAL 2383(mm) | free form MICIAL 2383(mg.ml−1) | free form MIC10(mg.ml−1) | sessile form MICIAL 2383(mg.ml−1) | sessile form MICIAL 468(mg.ml−1) | sessile form MICIAL 520(mg.ml−1) |
|---|---|---|---|---|---|---|
| O6 | 21.00 ± 1.40 | – | – | – | – | – |
| F6 | 36.00 ± 5.70* | 1.56 ± 0.00 | 2.60 ± 0.90 | 0.78 ± 0.00 | 0.78 ± 0.00 | 0.78 ± 0.00 |
| O14 | 23.00 ± 7.00 | – | – | – | – | – |
| F14 | 35.00 ± 2.80* | 1.56 ± 0.00 | 1.56 ± 0.00 | NIB | NIB | NIB |
| O19 | 28.00 ± 2.80 | – | – | – | – | – |
| F19 | 43.00 ± 1.40* | 1.25 ± 0.00 | 1.56 ± 0.90 | NIB | NIB | NIB |
Unpaired t-test was used to calculate statistically significant differences of IZ (mm) values between NLC and its respective controls (pure oils); *p < 0.05.
O6 (olibanum essential oil), O14 (salvia essential oil), and O19 (candeia oil) were the liquid lipids used as bioactive molecules in F6, F14, and F19 formulations, respectively. NIB, no inhibition of CJ in biofilms.