Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 21;12:495. doi: 10.1038/s41467-020-20804-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 8 — a Within 3 days period of differentiation, Myomerger-deficient C2C12 cells were labeled with either orange cell tracker or green cell tracker, and then treated with the hemifusion inhibitor lysophosphatidylcholine (LPC) in the differentiation medium (DM) for 16 h to accumulate myoblasts that are ready to fuse. LPC removal (‘LPC wash’) allowed the cells to undergo hemifusion and complete fusion. sMyomerger^26-84 in different concentrations was applied for 1 h at the time of LPC removal. In another experimental design, Myomerger-deficient C2C12 cells differentiated for 3 days (b, c) or w.t C2C12 cells differentiated for 2 days (d, e) were allowed to fuse without LPC block. b Quantification of synchronized fusion in the presence of different LPC concentrations and fusion extents observed at the same time post-differentiation for unsynchronized fusion (No LPC) for Myomerger-deficient C2C12 cells. c Representative images for the experiments in b taken for the No LPC, 0 and 10 μM sMyomerger^26-84 experiments. Arrows mark examples of fusion. Scale bar 50 μm. d W.t. C2C12 cells differentiated for 2 days, labeled with either orange cell tracker or green cell tracker and co-plated in the DM in the presence of different concentrations of sMyomerger^26-84. Fusion was scored 4 h after co-plating. e Representative images for the experiments in D taken for the 0 and 10 μM sMyomerger^26-84 experiments. Arrows mark examples of fusion. Scale bar 50 μm. b, d Fusion was quantified by assessing formation of multinucleated cells as the ratio of nuclei in the cells with ≥2 nuclei to the total number of nuclei. N, the number of randomly selected fields of view examined over three independent cell preparations for each condition, is indicated to the left of the box associated with that data. Box-and-whisker plots show median (center line), mean (blue line), 25–75th percentiles (box), 10–90th percentiles (whiskers), and 5–95th percentiles (solid circles). P-values were calculated using a two-tailed unpaired t-test.