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. 2021 Jan 21;11:2061. doi: 10.1038/s41598-021-81548-2

Figure 3.

Figure 3

Field relevant concentrations of neonicotinoids disrupted sleep behaviour. Sleep plots showing the total sleep achieved per 30 min bin over the 24 h period (zeitgeber time (ZT)) for flies exposed to 1, 10 or 50 µg/L of (a) imidacloprid, (b) clothianidin, (c) thiamethoxam or (d) thiacloprid. The number of (no.) of sleep episodes initiated in (e) IM, day (F3,114 = 1.2, p = 0.320) and night (F3,114 = 5.5, p = 0.001), (f) CLO, day (F3,120 = 11.5, p < 0.001) and night (F3,120 = 25.0, p < 0.001), (g) TMX, day (F3,124 = 1.1, p = 0.344) and night (F3,124 = 17.0, p < 0.001) or (h) TCL, day (F3,120 = 0.2, p = 0.872) and night (F3,120 = 3.0, p = 0.034). Mean length (in minutes) of sleep episodes initiated in (i) IM, day (F3,114 = 0.2, p = 0.889) and night (F3,114 = 4.5, p = 0.005), (j) CLO, day (F3,120 = 9.9, p < 0.001) and night (F3,120 = 21.8, p < 0.001), (k) TMX, day (F3,124 = 2.5, p = 0.061) and night (F3,124 = 15.7, p < 0.001) or (l) TCL, day (F3,120 = 5.2, p = 0.002) and night (F3,120 = 2.0, p = 0.121). Each data point represents a single fly, n = 28–32 flies per treatment. CLO and TMX were run together with one set of controls, whilst IM and TCL were run together with another control group.