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. 2021 Jan 21;11:1864. doi: 10.1038/s41598-021-81389-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cross-reactivity of commercial anti-human/mouse BCL6 antibodies with lymph node cells from influenza infected ferret. (a) Gating strategy to identify live lymphocytes in the ferret LN. Lymphocytes were identified by forward scatter area (FSC-A) and side-scatter area (SSC-A). Doublets were excluded by gating on single cells as determined by FSC-A versus FSC-H, and live cells were identified by viability dye exclusion. For each step, the parental population is indicated above the plot. (b) Representative plots of influenza-infected ferret lymph node cells stained with anti-BCL 6 (clone K112-91 and clone IG191E/A8), CD79a, CD4 and CD8 antibodies. The parental population is indicated above the plot. Blue box indicates GC B cells (BCL6+CD79a+) and red box indicates Tfh cells (BCL6+CD4+).