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. 2021 Jan 21;11:2034. doi: 10.1038/s41598-021-81598-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Nodulation of G. max cv. En1282 (nfr1 mutant soybean) inoculated with Bel2-5 ULP1 domain mutant strains. (a) The number of nodules formed on En1282 inoculated with wild-type and the mutants of predicted catalytic core of Bel2-5 ULP1 at 30 dai. Values represent mean ± SD (n = 15, from three independent inoculation tests). **p < 0.001 by two-tailed Student t-test compared to USDA61 wild-type. (b) Roots of En1282 inoculated with wild-type and the mutants of predicted catalytic core of Bel2-5 ULP1 were photographed at 30 dai. Scale bars: 1 cm.