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. 2021 Jan 21;12(1):105. doi: 10.1038/s41419-021-03411-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Heat map of HOXB4 RNA-seq results. All enriched features of the enriched gene sets, which were filtered and analyzed by GSEA analysis of HOXB4 RNA-seq data, were shown between the vector control (Vector) and HOXB4-overexpressing (HOXB4) HeLa cells. Gene expression values were represented as colors, where the range of colors (red, pink, light blue, dark blue) showed the range of expression values (high, moderate, low, lowest). n = 3 per group. b–g Expression of β-catenin and c-Myc. The mRNA levels of HOXB4, β-catenin, and c-Myc were detected by qRT-PCR in HOXB4-overexpressing HeLa (b) and C-33A (d) cells, as well as in HOXB4-depleted SiHa (f) cells. Data were normalized to GAPDH. The protein expression of HOXB4, total β-catenin, nuclear β-catenin, and c-Myc was assessed in HOXB4-modified HeLa (c), C-33A (e), and SiHa (g) cells by western blot and normalized to GAPDH and histone H3 (H3) respectively. h–j Inactivation of the Wnt/β-catenin reporter luciferase by HOXB4. HeLa (h), C-33A (i), or SiHa (j) cells transfected with the indicated plasmids were analyzed by β-catenin reporter luciferase assays (TOPFlash (TOP) and FOPFlash (FOP)).