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. 2021 Jan 21;11:2010. doi: 10.1038/s41598-021-81474-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Line HD1A is reactive to DAT₂. (A) Flow cytometry histogram of line HD1A stained with DAT₁-, DAT₂-, or DAT₃-, or mock-treated CD1b tetramers (CD1b-mock). (B) IFN-γ ELISPOT of line HD1A stimulated with monocyte-derived dendritic cells treated with DAT₁, DAT₂, or DAT_3, with or without anti-CD1b antibody or Isotype control. Error bars represent standard error of the mean (SEM) of triplicate wells. One representative experiment of three is shown.