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. 2021 Jan 21;7:9. doi: 10.1038/s41531-020-00150-x

Table 2.

Primary and secondary antibodies, their sources and dilutions.

1° Antibody Host Dilution Source 2° Antibody Dilution
Neuronal protein monoclonal HuC/D Mouse 1:50 16A11: Molecular probes Donkey anti-mouse Alexa fluor 350 IgG (H + L) 1:100
Vasoactive intestinal polypeptide polyclonal Rabbit 1:100 Sc-20727; Santa Cruz Biotechnology Horse FITC anti-rabbit IgG (H + L) 1:100
Neuronal nitric oxide synthase polyclonal Rabbit 1:500 AB5380: Millipore Goat FITC anti-rabbit IgG (H + L) 1:100
Tyrosine hydroxylase polyclonal Rabbit 1:500 P40101; Pel-Freez Biologicals, USA Biotinylated goat anti-rabbit-IgG (H + L) 1:200
Tyrosine hydroxylase polyclonal Chicken 1:400 AB76442, Abcam Goat anti-chicken Alexa fluor 488 IgG (H + L) 1:100
Choline Acetyltransferase polyclonal Goat 1:20 AB144P; Millipore Streptavidin 595 with biotinylated anti-goat IgG (H-L) made in rabbit 1:100 / 1:200
SOX-10 monoclonal Rabbit 1:100

ab155279

Abcam

Donkey anti-rabbit Alexa-fluor 594 IgG (H + L) 1:100
GFAP monoclonal Rabbit 1:200

345860

Merck Chemicals

Donkey anti-rabbit Alexa-fluor 594 IgG (H + L) 1:100
S100β monoclonal Rabbit 1:200

ab52642

Abcam

Donkey anti-rabbit Alexa-fluor 594 IgG (H + L) 1:100

α-Synuclein

monoclonal

Mouse 1:800

BD 610787

BD/Transduction

Donkey anti-mouse Alexa-fluor 594 IgG (H + L) 1:100