Table 2.
Primary and secondary antibodies, their sources and dilutions.
1° Antibody | Host | Dilution | Source | 2° Antibody | Dilution |
---|---|---|---|---|---|
Neuronal protein monoclonal HuC/D | Mouse | 1:50 | 16A11: Molecular probes | Donkey anti-mouse Alexa fluor 350 IgG (H + L) | 1:100 |
Vasoactive intestinal polypeptide polyclonal | Rabbit | 1:100 | Sc-20727; Santa Cruz Biotechnology | Horse FITC anti-rabbit IgG (H + L) | 1:100 |
Neuronal nitric oxide synthase polyclonal | Rabbit | 1:500 | AB5380: Millipore | Goat FITC anti-rabbit IgG (H + L) | 1:100 |
Tyrosine hydroxylase polyclonal | Rabbit | 1:500 | P40101; Pel-Freez Biologicals, USA | Biotinylated goat anti-rabbit-IgG (H + L) | 1:200 |
Tyrosine hydroxylase polyclonal | Chicken | 1:400 | AB76442, Abcam | Goat anti-chicken Alexa fluor 488 IgG (H + L) | 1:100 |
Choline Acetyltransferase polyclonal | Goat | 1:20 | AB144P; Millipore | Streptavidin 595 with biotinylated anti-goat IgG (H-L) made in rabbit | 1:100 / 1:200 |
SOX-10 monoclonal | Rabbit | 1:100 |
ab155279 Abcam |
Donkey anti-rabbit Alexa-fluor 594 IgG (H + L) | 1:100 |
GFAP monoclonal | Rabbit | 1:200 |
345860 Merck Chemicals |
Donkey anti-rabbit Alexa-fluor 594 IgG (H + L) | 1:100 |
S100β monoclonal | Rabbit | 1:200 |
ab52642 Abcam |
Donkey anti-rabbit Alexa-fluor 594 IgG (H + L) | 1:100 |
α-Synuclein monoclonal |
Mouse | 1:800 |
BD 610787 BD/Transduction |
Donkey anti-mouse Alexa-fluor 594 IgG (H + L) | 1:100 |