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. 2021 Jan 21;11:2048. doi: 10.1038/s41598-021-81735-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Silencing of E-cadherin induced a partial EMT state. (a) Wound healing assay was made after cells were treated with or without Dox (+Dox and −Dox, respectively) (t = 0 h). Cells were treated with Dox during 72 h and then scratch was made (t = 0 h for this experiment). Images were taken at t = 0 h and after 22 h of recovery (t = 22 h) (left). Graphic represents the relative wound closure area of at least four independent experiments (right). AU is the abbreviation for arbitrary units. T test. Different letters indicate significant differences. (b) Rescue experiment to regain the knockdown effect. Cells were incubated with Dox for 96 h. At this time point, Dox was removed and cells were maintained for another 96 h (4 days) only with culture medium. CDH1 and SNAI1 gene expression was evaluated by qPCR. Results are represented as mean ± SD (n = 5). AU is the abbreviation for arbitrary units. ANOVA with post hoc Tukey. Different letters indicate significant differences of groups compared to control condition (p < 0.01).