Table 2.
Colony counts (colony-forming units—CFU/ml) and their proportion to total cell counts determined by flow cytometry (‰) at different cultivation conditions and media.
| Temperature/ cultivation days | Ice-1 CFU (‰) |
Ice-2 CFU (‰) |
Ice-3 CFU (‰) |
Ice-1 CFU (‰) |
Ice-2 CFU (‰) |
Ice-3 CFU (‰) |
|---|---|---|---|---|---|---|
| NA | R2A | |||||
| 37 °C/ 2 | 5 (0.11) | 13 (0.23) | 78 (0.52) | 0 (0.00) | 5 (0.09) | 190 (1.25) |
| 20 °C/ 14 | 85 (1.82) | 150 (2.75) | 5,025 (33.17) | 1,001 (21.43) | 366 (6.72) | 10,900 (71.97) |
| 10 °C/ 28 | 523 (11.19) | 786 (14.42) | 10,567 (69.77) | 579 (12.40) | 850 (15.59) | 11,400 (75.27) |
| 5 °C/ 28 | 837 (17.92) | 1,097 (20.12) | 5,810 (38.36) | 740 (15.84) | 829 (15.21) | 16,775 (110.76) |
| RIDA | RIDA-an | |||||
|---|---|---|---|---|---|---|
| 37 °C/ 2 | 1 (0.02) | 2 (0.04) | 99 (0.65) | 1 (0.02) | 1 (0.02) | 33 (0.21) |
| 20 °C/ 14 | 45 (0.96) | 118 (2.16) | 55 (0.36) | 4 (0.09) | 4 (0.06) | 230 (1.52) |
| 10 °C/ 28 | 43 (0.92) | 81 (1.49) | 253 (1.67) | 14 (0.30) | 35 (0.63) | 479 (3.16) |
| 5 °C/ 28 | 66 (1.41) | 96 (1.76) | 230 (1.52) | 15 (0.31) | 26 (0.48) | 553 (3.65) |
| MEA | RIDA-Y&M-an | |||||
|---|---|---|---|---|---|---|
| 20 °C/ 14 | 32 (0.68) | 80 (1.47) | 736 (4.86) | + | + | + |
| 10 °C/ 28 | 398 (8.53) | 39 (0.71) | 331 (2.19) | + | + | + |
| 5 °C/ 28 | 41 (0.88) | 56 (1.03) | 401 (2.65) | + | + | + |
| TWA | PWA | |||||
|---|---|---|---|---|---|---|
| 20 °C/ 14 | 155 (3.32) | 145 (2.66) | 8,520 (56.25) | 103 (2.19) | 326 (5.99) | 12,038 (79.48) |
| 10 °C/ 28 | 93 (1.98) | 155 (2.84) | 4,280 (28.26) | 1,242 (26.59) | 1,957 (35.90) | 25,350 (167.37) |
| 5 °C/ 28 | 110 (2.35) | 577 (10.58) | 5,983 (39.50) | 295 (6.32) | 810 (14.86) | 12,525 (82.70) |
In bold—highest value; RIDA RIDACOUNT total aerobic count; RIDA-Y&M RIDACOUNT yeast&mold rapid; an anaerobic, + presence, not countable.