Table 1.
A list of all primers tested in this study.
| Primer/probe | Sequence (5′–3′) | Primer/probe length | Amplicon size |
|---|---|---|---|
| For fliC gene | |||
| Eco fliC F1 | TTGATGAAATTGACCGCGTATCCGGCCAGACC | 32 nt | 172 |
| Eco fliC R1 | ATTTACGTTAAAGCCATTCAGACCCAGAGTAT | 32 nt | |
| Eco fliC F2 | TGCAGGCAACTTGACGACTAACAACGCTGGTAG | 33 nt | 169 |
| Eco fliC R2 | TTAACGGAGCTACTGGAGTGGTTGTCGCAGG | 31 nt | |
| Eco fliC F3 | ATGAAATTGACCGCGTATCCGGCCAGACCCAG | 32 nt | 169 |
| Eco fliC R3 | ACCATTTACGTTAAAGCCATTCAGACCCAGAG | 32 nt | |
| Eco fliC F4 | CTTTATGATCTGAAAACCGAAAATACCTTGTT | 32 nt | 266 |
| Eco fliC R4 | TCTTTTGTGGTGTAAGAACCATTAACAGATTT | 32 nt | |
| For rfbE gene | |||
| Eco rfbE F1 | AGCTTTGTTAGCGTTAGGTATATCGGAAGGAGA | 33 nt | 216 |
| Eco rfbE R1 | ACATGGATGTCCGTATAAATGGACACACATAAT | 33 nt | |
| Eco rfbE F2 | AGCTTTGTTAGCGTTAGGTATATCGGAAGGAGATG | 35 nt | 217 |
| Eco rfbE R2 | TCACATGGATGTCCGTATAAATGGACACACATAAT | 35 nt | |
| Eco rfbE F3 | GTTAACTGTTATGTTGTACTGCTTCATTTTTA | 32 nt | 187 |
| Eco rfbE R3 | GAGTCCAGACATTCATTTACATATTCTTTTTC | 32 nt | |
| Eco rfbE F4 | AAAGAGAGGAATTAAGGAATCACCTTGCAGAT | 32 nt | 169 |
| Eco rfbE R4 | ATTCGATAGGCTGGGGAAACTAGGTAAATTAA | 32 nt | |
| For stx gene | |||
| Eco stx1 F1 | GGAACCTCACTGACGCAGTCTGTGGCAAGAGC | 32 | 179 |
| Eco stx1 R1 | CAACCTTCCCCAGTTCAATGTAAGATCAACAT | 32 | |
| Eco stx1 F2 | CCTCACTGACGCAGTCTGTGGCAAGAGCGATG | 33 | 179 |
| Eco stx1 R2 | TACTCAACCTTCCCCAGTTCAATGTAAGATCA | 32 | |
| Eco stx2 F1 | GTGGCCGGGTTCGTTAATACGGCAACAAATAC | 32 | 179 |
| Eco stx2 R1 | TGAAACCAGTGAGTGACGACTGATTTGCATTC | 32 | |
| Eco stx2 F2 | ATATCTCAGGGGACCACATCGGTGTCTGTTAT | 32 | 180 |
| Eco stx2 R2 | GTAGAAAGTATTTGTTGCCGTATTAACGAACC | 32 | |
FP forward primer, RP reverse primer, nt nucleotides.