Table 3.
Selectivity evaluation of RPA-AGE and RPA-LFA using rfbE and fliC gene targets.
| S. no | Bacteria | Serotype | Strain | Classification | rfbE gene | fliC gene | stx gene | |||
|---|---|---|---|---|---|---|---|---|---|---|
| RPA-AGE | RPA-LFA | RPA-AGE | RPA-LFA | RPA-AGE | RPA-LFA | |||||
| 1 | E. coli | O157:H7 | ATCC 43888 | STEC | + | + | + | + | + | + |
| 2 | E. coli | O157:H7 | ATCC 43895 | EHEC (STEC) | + | + | + | + | + | + |
| 3 | E. coli | O157:H7 | ATCC 8740 | EHEC (STEC) | + | + | + | + | + | + |
| 5 | E.coli | O26:H- | NCTC 8620 | EPEC | – | – | + | + | – | – |
| 6 | E. coli | O6 biotype 1 | ATCC 25922 | – | – | + | + | – | – | |
| 7 | E. coli | O1:K1:H7 | NCTC 9001 | ExPEC or APEC | – | – | + | + | – | – |
| 8 | E. coli | O129:H11 | ATCC 23542 | EPEC | – | – | – | – | – | – |
| 9 | E. coli | NCTC 11560 | – | – | – | – | – | – | ||
| 4 | E. coli | ATCC 35218 | – | – | – | – | – | – | ||
| 10 | E. coli | O103 | NCTC 8196 | EHEC, STEC non-O157 | – | – | – | – | – | – |
| 11 | E. coli | W3110 | ATCC 27325 | – | – | – | – | – | – | |
| 12 | Bacillus cereus | ATCC 11778 | – | – | + | + | – | – | ||
| 13 | Klebsiella pneumonia | ATCC 13883 | – | – | + | + | – | – | ||
| 14 | Pseudomonas aerouginosa | ATCC 27853 | – | – | + | + | – | – | ||
| 15 | Shigella dysenteria | NCTC 4837 | – | – | – | – | – | – | ||
| 16 | Listeria monocytogenes | ATCC 19115 | – | – | – | – | – | – | ||