Table 2.
Estimated olfactory bulb α-synuclein number and density.
| Cases | DxAP | Total markers counted | Number of sections | Number of sampling sites | Coefficient of error (Gundersen), m = 1 | Counting frame area (XY) (µm²) | Sampling grid area (XY) (µm²) | Estimated population using mean section thickness | Measured volume (mm³) | Density (cell/mm3) |
|---|---|---|---|---|---|---|---|---|---|---|
| 5 | PD | 499 | 6 | 236 | 0.05 | 900 | 250,000 | 829,533.44 | 14.39 | 57,666.56 |
| 7 | PD | 481 | 4 | 70 | 0.05 | 900 | 1,000,000 | 3,193,458.75 | 17.73 | 180,129.32 |
| 8 | PD | 499 | 5 | 62 | 0.05 | 900 | 1,000,000 | 3,342,852.25 | 15.70 | 212,874.43 |
| 9 | PD | 413 | 5 | 61 | 0.05 | 900 | 1,000,000 | 2,718,442.00 | 15.70 | 173,182.26 |
| 10 | PD | 1744 | 3 | 196 | 0.03 | 900 | 250,000 | 2,938,421.25 | 11.86 | 247,775.67 |
| 11 | PD | 395 | 4 | 77 | 0.06 | 900 | 1,000,000 | 2,598,875.75 | 19.65 | 132,230.04 |
| 12 | PD | 203 | 7 | 51 | 0.07 | 900 | 1,000,000 | 1,333,214.38 | 11.52 | 115,769.61 |
| 14 | PD | 460 | 6 | 93 | 0.05 | 900 | 1,000,000 | 3,032,370.50 | 22.55 | 134,480.95 |
| 15 | PD | 296 | 5 | 75 | 0.06 | 900 | 1,000,000 | 1,948,283.75 | 18.15 | 107,339.32 |
| 16 | PD | 508 | 3 | 73 | 0.07 | 900 | 250,000 | 854,206.94 | 4.29 | 198,906.27 |
| 17 | PD | 648 | 3 | 108 | 0.05 | 900 | 250,000 | 1,088,870.25 | 6.40 | 170,003.69 |