Fig. 7. GATA2 primes the activation-inducible enhancers to respond to antigenic stimulation.

a TF binding motifs enriched in the overlapped regions between the regions with the activation-induced H3K27ac modification and the regions with the activation-induced accessibility (highlighted). H3K27ac ChIP-seq and Omni-ATAC-seq were prepared from resting (UN) and activated mast cells (ST). b The number of GATA2 and MITF binding motifs (left panel) and occupancy (total bound sites and reads per gene, middle panel) and normalized bound sites and reads per kb per enhancer (right panels) (n = 2 biologically independent samples). c Flow cytometry analysis of the WT and Gata2 KO BMMCs at 5 days after 4-OHT treatment. d Calculated average reads at enhancers and RNA transcripts for WT and Gata2 KO MCs under resting or activated conditions. Two biological samples for each NGS sequencing under each condition. e Representative tracks for cells and treatments indicated in d. The IGV tracks are generated from one biological sample, representing two biological replicates with similar patterns. Middle line inside each box represents the median, upper and lower bounds of the box represent the third and first quartiles, respectively. Whiskers represent 1.5 times of the interquartile range. P-values were calculated by a two-tailed student’s t test without adjustments.