Figure 3.

Mouse Studies Reveal a Role of KDM4B in the Anatomy of the Brain

(A) Construction of the Kdm4b^{tm1a(EUCOMM)Wtsi} allele.

(B) Histograms for three heterozygous Kdm4b mice showing percentage difference relative to 40 WTs. The overlapping dots show actual data points. To present areas and lengths on the same scale, the square root of areas was used. Inserts are full dot and box plots of WT (black) versus Kdm4b^+/− mice (red) for each of the four significant parameters (total brain area, lateral ventricle area, corpus callosum height, dentate gyrus length).

(C and D) Left: schematic representation of a section at Bregma +0.98 mm and Bregma −1.34 mm. Colored regions indicate the presence of at least one significant parameter within the brain region at the 0.05 level. White coloring indicates a p value higher than 0.05 and gray shows there is not enough data to calculate a p value. Right: illustrating example of WT and Kdm4b^+/− brain images in coronal sections double-stained for Nissl and Luxol at Bregma −1.34 (C) and Bregma −2.06 mm (D).

(E) Dot plot of the coronal section at Bregma −2.06 mm showing further characterization of the hippocampus.

(F) Body weight of seven Kdm4b^+/− and 771 baseline control mice.

(G) Distance traveled in cm in the open-field arena shown in sessions of 2 min each.

(H) Number of rears measured in the open-field arena.

(I) Distance and time in the open-field arena expressed in percentages.

(J) Resting time and average speed in the open-field test.

(K) Average speed in the center of the open-field arena, latency to enter the center, and the number of entries to the center.