Figure 6.

Defective VPS4A Function Affects Centrosome Numbers and Mitotic Spindle Organization

Confocal microscopy analysis was performed in synchronized skin fibroblasts from subjects with VPS4A mutations and control cells. Images are representative of three stages of the cell cycle (A), interphase (B), metaphase, and anaphase-telophase. Cells were stained using antibodies against pericentrin (centrosome marker) and α-tubulin (microtubules and mitotic spindle); chromosomes with DAPI. Scale bars represent 15 μm. The corresponding graphs show the mean ± SEM of 6 separate counts (≥25 cells/line each) for a total of 200 cells/line scored. p values were calculated by one-way ANOVA with Tukey’s correction for multiple testing.