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. 2019 Dec 22;40(12):2374–2386. doi: 10.1177/0271678X19892777

Figure 4.

Figure 4.

EPC transplantation attenuated astrocyte proliferation and reduced astrocyte-derived C3 expression in the mouse brain. (a) Representative images of double fluorescence immunostaining of C3 (green) with CD31 (red; a, e, i), MAP2 (red; b, f, j), GFAP (red; c, g, k) and Iba1 (red; d, h, i) in mice brain at 3 days after tMCAO. Scale bar=50 μm. (b) Quantifications of the numbers of C3+, GFAP+ and C3+/GFAP+ cells among groups. The data in Sham group were normalized to 1. Data are represented as mean ± SD. n=3–4 per group. *p<0.05. (c) Representative images of double fluorescence immunostaining of C3 (green) with CD31 (red; a, e, i), MAP2 (red; b, f, j), GFAP (red; c, g, k) and Iba1 (red; d, h, i) in mice brain at 14 days after tMCAO. Scale bar=50 μm. (d) Quantifications of the numbers of C3+, GFAP+ and C3+/GFAP+ cells among groups. The data in Sham group were normalized to 1. Data are represented as mean±SD. n=3–4 per group. *p<0.05.