Table 1.

Measured Dissociation and Inhibition Constants of Six Sulfonamides to hCA I and hCA II Obtained Using Native Mass Spectrometry and a Stopped Flow Kinetic Inhibition Assay, Respectively

Inhibitor	hCA I		hCA II
Inhibitor	Native MS	Stopped Flow	Native MS	Stopped Flow
Ethoxzolamide	0.014 ± 0.002 (19)	0.025^a (21), 0.009^b (22)	0.010 ± 0.001 (19)	0.008^a (22)
Brinzolamide	1.06 ± 0.05 (19)	0.73 ± 0.04^a (23)	0.005 ± 0.001 (19)	0.003^a (23)
Furosemide	0.055 ± 0.005 (19)	0.062^a (19)	0.098 ± 0.009 (19)	0.065^a (23)
Dichlorophenamide	1.3 ± 0.1 (19)	1.2^a (24)	0.027 ± 0.002 (19)	0.038^a (24)
Indapamide	9.2 ± 0.3 (19)	51.9^a (25)	3.22 ± 0.20 (19)	2.52^a (25)
Acetazolamide	0.24 ± 0.02 (19)	0.25^a (21)	0.015 ± 0.001 (19)	0.012^a (21)

University of Florence stopped flow kinetic inhibition constant measurements (μM) (21,23, 24, 25).

University of Florida buffer indicator kinetic method measurements (22).