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Preparation of samples in petrolatum provides the most stable method for CT imaging. (A) Representative images of Spirodela polyrhiza condition under different preparation methods over time. (B) Percentage change in frond area after 1 and 6 h under different preparation methods, * significant difference in mean area from start point p < 0.05, n.s. = No Significance in mean area change from start, n = 32 ANOVA with a Tukeys multiple comparison test.
