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. 2021 Jan 8;11:617830. doi: 10.3389/fpls.2020.617830

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Copyright © 2021 Jones, Atkinson, Ware, Sturrock, Bishopp and Wells.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MicroCT imaging confirms that the extracellular spaces seen in root sections are filled with gas (A)μCT micrograph of Spirodela intermedia root embedded in petrolatum, with air bubble inclusions (ab). (B) Confocal image of a Spirodela intermedia root section, 150 μm thick, stained with calcofluor (C) Segmentation of (A) using the method described in Figure 3, (D) Recolored root section (B) showing the classification of extracellular spaces based on segmentation in (B), Scale Bars: (A,C) = 200 μm (C,D) = 50 μm.