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. 2021 Jan 8;11:617830. doi: 10.3389/fpls.2020.617830

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Copyright © 2021 Jones, Atkinson, Ware, Sturrock, Bishopp and Wells.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Buoyant Spirodela polyrhiza turions contain a higher percentage of gaseous extracellular space than those that are submerged. (A–C) Mid slice of three μCT micrographs of stacks of three turions, collected under differing conditions. (D–F) Segmentation of images from (A) following steps in Figure 3. Green: Tissue, Blue: Gas, White: Starch (A,D) Turions floating at surface, (B,E) Turions sunk to bottom of flask, (C,F) cold treated turions showing petrolatum (p), Starch (s), Tissue (t), and gas spaces (gs). (G) Expanded micrograph of Cold treated turion. (H) Expanded segmentation of micrograph of Cold treated turion showing petrolatum (p), Starch (s), Tissue (t), and gas spaces (gs) recolored as determined by segmentation. (I) Composition values of each turion set under each treatment as determined by processing and analysis pipeline. Dots represent data points, horizontal line the mean value. Scale Bars (A–F): 1 mm, (G,H): 0.1 mm.